Charles E. Glatz
Professor
2162B Sweeney Hall
Iowa State University
Ames, IA 50011-2230
Phone (515)294-8472
Fax (515)294-2689
cglatz
iastate.edu
Teaching/Office Hours Schedule
Education
PhD, ChE, University of Wisconsin - Madison, 1975
BS, ChE, University of Notre Dame, 1971
Honors and Awards
Regents' Award for Faculty Excellence, 2007
Boylan Award for Outstanding Research, ISU College of Engineering, 2003
Iowa Academy of Science, Distinguished Iowa Scientist, 1997
Visiting Professor, University of Colorado, Boulder, 1994
Outstanding Teacher Award, College of Engineering, 1990
Honorary Research Fellow, University of Hull and University College London, 1982-83
Research Interests
Biochemical separations; protein recovery from plants; aqueous oil extraction; kinetics of protein precipitation; protein crystallization; membrane processing; protein extraction; fermentation.
Other Information
Editorial Board, Separation Science and Technology, 1996-present
American Institute of Chemical Engineers
American Chemical Society
American Society of Engineering Education
Research Projects
Bioprocessing is the common thread running through our research. Most of these projects focus on separations problems, and most are collaborative efforts that involve biochemists, microbiologists, genetic engineers, and chemists. In carrying out our work, we are able to use not only our laboratories and those of our collaborators but also the instrumentation facilities operated by the ISU Biotechnology Council.
Recovery of Recombinant Proteins
In the downstream processing of products from biological sources, the desired product frequently is a protein that must be recovered from a dilute aqueous stream. We are studying recovery of such proteins by a variety of separation methods including precipitation, aqueous two-phase extraction, chromatography, and membrane separations such as ultrafiltration. One project is aimed at protein recovery from transgenic plants where we apply a combination of biological (host selection and tissue specific targeted expression), chemical (choice of separating agent), and engineering (equipment mode of operation) approaches to developing separation strategies. By combining proteomics, aqueous phase partitioning, and statistical modeling we are developing design tools for protein recovery. Specific product applications of our work include proteins/peptides with useful material properties.
A second project is biomass removal and extracted protein recovery using microfiltration. Here the emphasis is on capturing the fundamentals of flux limitations, treatments to eliminate the limitations, and mathematically modeling the phenomena controlling flux to provide for reliable scale up of processes from lab data.
Aqueous Extraction of Soy Oil
Our aim in this project is to initiate development of a process to use enzymatic treatments to improve the recovery of oil from soybeans without the use of organic solvents - i.e. a “green” processing route that replaces hexane with water. The questions to be addressed include oil release, matrix degradation, emulsion breakage, and byproduct potential.
Recent Publications
Graves, K., G. Rozeboom, M. Heng, and C. E. Glatz. Biotechnol. Bioengr., 94, 346-352, 2006. Broth conditions determining specific cake resistance during microfiltration of Bacillus subtilis.
Glatz, C. E., R. González, M. E. Huba, S. K. Mallapragada, B. Narasimhan, P. J. Reilly, K. P. Saunders, J. V. Shanks. Biotechnol. Prog., 22, 173-178, 2006. Problem-based learning biotechnology courses in chemical engineering.
Zhong, Q. and C. E. Glatz. J. Agric. Food Chem., 54, 3181-3185, 2006. An enzymatic assay method for evaluating the lipase activity in complex extracts from transgenic corn seed.
Zhong, Q., Gu, Z., and C. E. Glatz. J. Agric. Food Chem., 54, 8086-8092, 2006. Extraction of recombinant dog gastric lipase from transgenic corn.
Gu, Z. and C. E. Glatz. J. Chromatogr. B, 845, 38-50, 2007. Aqueous two-phase extraction for protein recovery from corn extracts.
Gu, Z. and C. E. Glatz. Sep. Sci. Technol., 42, 1195-12-3, 2007. Recovery of recombinant dog gastric lipase from corn endosperm extract
Gu, Z. and C. E. Glatz. Biotechnol. Bioengr., 97, 1159-1169, 2007. A method for three dimensional protein characterization and its application to a complex plant (corn) extract.
Q. Zhong, L. Xu, C. Zhang, and C. E. Glatz, Appl. Microbiol. Biotechnol., 76, 607–613, 2007. Purification of recombinant aprotinin from transgenic corn germ fraction using ion exchange and hydrophobic interaction chromatography.
R. Morales-Chabrand, H.-J. Kim, C. Zhang, C. E. Glatz, S. Jung. J. Am. Oil Chem. Soc., 85, 383–390, 2008. Destabilization of the emulsion formed during aqueous extraction of soybean oil.
M. A. Aspelund, M. Heng, G. Rozeboom, C. E. Glatz. J. Mem. Sci. 324,198-208, 2008. Improving permeate flux and product transmission in the microfiltration of a bacterial cell suspension by flocculation with cationic polyelectrolytes.
J. M. L. N. de Moura, K. Campbell, A. Mahfuz, S. Jung, C. E. Glatz and L. Johnson. J. Am. Oil Chem. Soc. Published on-line 2008. DOI 10.1007/s11746-008-1199-9. Enzyme-assisted aqueous extraction of oil and protein from soybeans and cream de-emulsification.
D. J. Cookman, C. E. Glatz. Bioresource Technology, 100, 2012–2017, 2009. Extraction of protein from distiller’s grain.
L. Xu and C. E. Glatz. J. Chromatogr. A, 1216, 274–280, 2009. Predicting protein retention time in ion-exchange chromatography based on three-dimensional protein characterizations.
