{"id":778,"date":"2022-08-04T21:10:48","date_gmt":"2022-08-04T21:10:48","guid":{"rendered":"https:\/\/www.cbe.iastate.edu\/mollykoz\/?p=778"},"modified":"2022-08-16T17:40:49","modified_gmt":"2022-08-16T17:40:49","slug":"dna-directed-patterning-for-versatile-validation-and-characterization-of-a-lipid-based-nanoparticle-model-of-sars-cov-2","status":"publish","type":"post","link":"https:\/\/www.cbe.iastate.edu\/mollykoz\/publications\/dna-directed-patterning-for-versatile-validation-and-characterization-of-a-lipid-based-nanoparticle-model-of-sars-cov-2\/","title":{"rendered":"DNA-directed patterning for versatile validation and characterization of a lipid-based nanoparticle model of SARS-CoV-2"},"content":{"rendered":"<p><strong>M Kozminsky<\/strong>, TR Carey, LL Sohn. &#8220;<a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/advs.202101166\">DNA\u2010Directed Patterning for Versatile Validation and Characterization of a Lipid\u2010Based Nanoparticle Model of SARS\u2010CoV\u20102<\/a>.&#8221; <i>Advanced Science<\/i>\u00a08.23 (2021): 2101166.<!--more--><\/p>\n<p>Abstract: Lipid-based nanoparticles have been applied extensively in drug delivery and vaccine strategies and are finding diverse applications in the coronavirus disease 2019 (COVID-19) pandemic\u2014from vaccine-component encapsulation to modeling the virus, itself. High-throughput, highly flexible methods for characterization are of great benefit to the development of liposomes featuring surface proteins. DNA-directed patterning is one such method that offers versatility in immobilizing and segregating lipid-based nanoparticles for subsequent analysis. Here, oligonucleotides are selectively conjugated onto a glass substrate and then hybridized to complementary oligonucleotides tagged to liposomes, patterning them with great control and precision. The power of DNA-directed patterning is demonstrated by characterizing a novel recapitulative lipid-based nanoparticle model of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)\u2014S\u2013liposomes\u2014that presents the SARS-CoV-2 spike (S) protein on its surface. Patterning a mixture of S\u2013liposomes and liposomes that display the tetraspanin CD63 to discrete regions of a substrate shows that angiotensin-converting enzyme 2 (ACE2) specifically binds to S\u2013liposomes. Subsequent introduction of S\u2013liposomes to ACE2-expressing cells tests the biological function of S\u2013liposomes and shows agreement with DNA-directed patterning-based assays. Finally, multiplexed patterning of S\u2013liposomes verifies the performance of commercially available neutralizing antibodies against the two S variants. Overall, DNA-directed patterning enables a wide variety of custom assays for the characterization of any lipid-based nanoparticle.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>M Kozminsky, TR Carey, LL Sohn. &#8220;DNA\u2010Directed Patterning for Versatile Validation and Characterization of a Lipid\u2010Based Nanoparticle Model of SARS\u2010CoV\u20102.&#8221; Advanced Science\u00a08.23 (2021): 2101166.<\/p>\n","protected":false},"author":3545,"featured_media":779,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[3],"tags":[],"class_list":["post-778","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/posts\/778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/users\/3545"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/comments?post=778"}],"version-history":[{"count":1,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/posts\/778\/revisions"}],"predecessor-version":[{"id":780,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/posts\/778\/revisions\/780"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/media\/779"}],"wp:attachment":[{"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/media?parent=778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/categories?post=778"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cbe.iastate.edu\/mollykoz\/wp-json\/wp\/v2\/tags?post=778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}